Activated Protein C Antibody

Catalog Number

250212

Unit

0.2 mg

Price

$570.00

Isotype

Applications

Species reactivity

Format

Each vial contains 0.2 mg IgG in 0.2 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-A/G affinity chromatography.

Description

Protein C is a vitamin K-dependent serine protease produced in the liver and made up of 2 polypeptide chains. The 62kDa proenzyme is activated by thrombin and the active enzyme cleaves factor Va and VIIIa and thus inhibits blood coagulation. The molecular weight of the active enzyme is 55kDa and the normal concentrations in human plasma is approximately 1-3 ng/ml because of the very fast turnover, the proenzyme concentration is approximately 3 µg/ml. The activated protein C (APC) is inhibited by members of the serine protease inhibitor (serpin) family, og which alpha1-antitrypsin (AAT) and protein C inhibitor (PCI) are the most important.   This antibody is specific for a conformation-dependent neoepitope that is expressed in activated protein C upon complex-formation with alpha1-antitrypsin. No reaction is seen to non-complexed alpha1-antitrypsin and weak cross-reaction with protein C zymogen. The antibody specificity is calcium dependent.

Alternate Names

Autoprothrombin IIA;  Anticoagulant protein C;  Blood coagulation factor XIV

Accession No.

P04070

Clonality

7

Antigen

Recombinant form of human activated protein C (Drotrecogin alfa, Xigris)

Application Notes

Product Cat. No. 250212 reacts strongly with APC-AAT complexes in ELISA. It can be used in sandwich ELISA in combination with a polyclonal anti-protein C antiserum. Note, that the conformational neoepitope expressed in the APC-AAT complex can also be expressed in APC coated directly onto a high-binding microtiter plate.

E: 1:500

Storage

Store at 4°C. Product is guaranteed 6 months from the date of shipment.