CD14 (HI221) Antibody

Catalog Number

251003

Unit

2 ml

Price

$275.00

Isotype

Applications

Species reactivity

Format

Each vial contains 2 ml IgM (100 tests) in PBS pH7.4, 0.1% BSA with 0.09% sodium azide. Antibody was purified by hydroxyapatite chromatography.

Description

The CD14 (HI221) antibody recognizes a 53-55 kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein, which is found on cells of myelomonocytic lineage including monocytes (strongly), granulocytes (weakly) and also on most tissue macrophages. CD14 antigen is also present on reticular dendritic cells, some Langerhans cells, and B lymphocytes (weakly), but is absent from T lymphocytes, NK cells, erythrocytes and platelets. CD14 functions as a high affinity receptor for the complex of lipopolysaccharide (LPS-endotoxin) and LPS-binding protein (LBP), and is the major receptor involved in the lethal response to both endotoxin and Gram-negative bacteria. In addition to membrane CD14, soluble form of CD14 can be detected in serum and tissue culture supernatants of cells transfected with CD14.

Product Citations

Tadamitsu K. et al., eds. 1997. Leucocyte Typing VI: White Cell Differentiation Antigens. P49-52, 113-114  Garland Publishing, Inc., New York.

Alternate Names

Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; Monocyte differentiation antigen CD14, urinary form; Monocyte fiferentiation antigen CD14, membrane-bound form; CD14

Accession No.

P08571
NP_000582

Clonality

HI221

Application Notes

FACS with whole blood: 100 μl blood + 20 μl antibody; FACS with cells: 10E6 cells + 20 μl antibody. 

FACS: 1:200-1:1,000

Storage

Store at 4°C. Product is guaranteed 6 months from the date of shipment.