CAM Ligands

The term ‘PDGF’ refers to a family of disulphide bond linked dimeric isoforms that act as autocrine and paracrine growth factors and are released by platelets. They act as potent mitogens for almost all mesenchymally-derived cells. Aberrant expression is involved in certain cancers, fibroproliferative disorders, and atherosclerosis. PDGF proteins also contribute to wound healing and neural regeneration. There are four members of the PDGF family: PDGF A, PDGF B, PDGF C and PDGF D.

L-Selectin belongs to a family of divalent cation dependent carbohydrate-binding glycoproteins or adhesion molecules. L-Selectin is expressed constitutively on lymphocytes, monocytes and granulocytes and interacts specifically with carbohydrate groups on activated endothelial cells. L-Selectin is shed by proteolytic cleavage and circulating levels in biological fluids are used as an indicator of various pathological conditions. Recombinant human L-selectin comprises a 294 amino acid fragment (39-332) corresponding to the mature L-Selectin extracellular domain and is expressed in E.

Hepatocyte growth factor (HGF-A) is a potent mitogen for mature parenchymal hepatocyte cells, is a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. It has no detectable protease activity. Hepatocyte growth factor is a dimer of an alpha chain and a beta chain linked by a disulfide bond. Defects in this protein are the cause of deafness autosomal recessive type 39 (DFNB39), a form of profound prelingual sensorineural hearing loss.

E-Selectin is a cell-surface glycoprotein which plays role in immunoadhesion. E-Selectin mediates the adhesion of blood neutrophils in cytokine-activated endothelium through interactions with PSGL1/SELPLG. It also plays a role in capillary morphogenesis. Recombinant human E-selectin comprises a 379 amino acid fragment (179-557) corresponding to the cysteine rich complement control region and is expressed in E. coli with an amino-terminal hexahistidine tag.