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GTP-binding Proteins

KRAS 293 Cell Lysate

$392.00
SKU: 408247
Antigen standard for v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC201958, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
List price: $0.00
Image 
KRAS lysate validation: The KRAS 293 Cell Lysate (lane B) and Control Vector Cell Lysate (lane A) are used in Western blot to detect tagged-KRAS with a DDK Antibody.
Subcategory 
Cell Lysate
Price: $392.00
Protein Family 
Enzymes
GTP-binding Proteins
Pathway and Disease 
Signal Transduction
Cell Motility
Cell Communication
Immune System
Endocrine System
Nervous and Sensory Systems
Development and Behavior
Cancers
ORF Clone URL 
http://www.origene.com/Human_ORF/RC201958.aspx
Format 
This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Applications 
ELISA
WB
IP
Alternate Names 
Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog; transforming protein p21; c-Kirsten-ras protein; K-ras p21 protein; oncogene KRAS2;; KRAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; RASK2
Application Notes 
WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Storage 
Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Release Date 
Thursday, December 13, 2012
Unit 
0.1 mg
Accession No.  
NP_004976

HRAS 293 Cell Lysate

$392.00
SKU: 401480
Antigen standard for v-Ha-ras Harvey rat sarcoma viral oncogene homolog (HRAS), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC216409, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
List price: $0.00
Image 
HRAS lysate validation: The HRAS 293 Cell Lysate (lane B) and Control Vector Cell Lysate (lane A) are used in Western blot to detect tagged-HRAS with a DDK Antibody.
Subcategory 
Cell Lysate
Price: $392.00
Protein Family 
GTP-binding Proteins
Transporters
Pathway and Disease 
Signal Transduction
Cell Motility
Cell Communication
Immune System
Endocrine System
Nervous and Sensory Systems
Development and Behavior
Cancers
ORF Clone URL 
http://www.origene.com/Human_ORF/RC216409.aspx
Format 
This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Applications 
ELISA
WB
IP
Alternate Names 
c-has/bas p21 protein; c-ras-Ki-2 activated oncogene; transformation gene: oncogene HAMSV; Ha-Ras1 proto-oncoprotein; Ras family small GTP binding protein H-Ras; p19 H-RasIDX protein; transforming protein p2; HRAS; C-BAS/HAS; C-H-RAS; C-HA-RAS1; CTLO; H-RASIDX; HAMSV; HRAS1; K-RAS; N-RAS; RASH1
Application Notes 
WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Storage 
Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Release Date 
Thursday, December 13, 2012
Unit 
0.1 mg
Accession No.  
NP_005334

NRAS 293 Cell Lysate

$392.00
SKU: 400852
Antigen standard for neuroblastoma RAS viral (v-ras) oncogene homolog (NRAS) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC202681, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
List price: $0.00
Image 
NRAS lysate validation: The NRAS 293 Cell Lysate (lane B) and Control Vector Cell Lysate (lane A) are used in Western blot to detect tagged-NRAS with a DDK Antibody.
Subcategory 
Cell Lysate
Price: $392.00
Protein Family 
GTP-binding Proteins
Pathway and Disease 
Signal Transduction
Cell Motility
Cell Communication
Immune System
Endocrine System
Nervous and Sensory Systems
Development and Behavior
Cancers
ORF Clone URL 
http://www.origene.com/Human_ORF/RC202681.aspx
Format 
This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Applications 
ELISA
WB
IP
Alternate Names 
v-ras neuroblastoma RAS viral oncogene homolog; N-ras protein part 4; transforming protein N-Ras; GTPase NRas; NRAS; ALPS4; N-ras; NRAS1; NS6
Application Notes 
WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Storage 
Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Release Date 
Thursday, December 13, 2012
Unit 
0.1 mg
Accession No.  
NP_002515

Tubulin-beta Antibody

$245.00
SKU: 251819
Tubulin is a major cytoskeleton protein that has five distinct forms designated alpha, beta, gamma, delta and epsilon tubulin. The alpha and beta tubulins form a heterodimer that polymerize into the cylindrical microtubule fibers. Both alpha and beta tubulin isomer can be modified by addition of a C-terminal tyrosine residue. This modification may influence polymerization rates. The gamma tubulin isomer is localized to centrosomes which compose the heart of the microtubule organizing center from which microtubule fibers emanate.
List price: $0.00
Price: $245.00

RhoA Antibody

$350.00
SKU: 251549
Transforming protein RhoA (RhoA) regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. RhoA serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RhoA is essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
List price: $0.00
Price: $350.00

NRas Antibody

$245.00
SKU: 251515
GTPase NRas (NRas) binds GDP/GTP and possess intrinsic GTPase activity. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP). Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML). JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia.
List price: $0.00
Price: $245.00

ARF6 Antibody

$280.00
SKU: 251514
ADP-ribosylation factor 6 (ARF6) is a GTP-binding protein involved in protein trafficking. ARF6 regulates endocytic recycling and cytoskeleton remodeling, and modulates vesicle budding and uncoating within the Golgi apparatus. ARF6 functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.Arf6 is involved in the regulation of dendritic spine development.
List price: $0.00
Price: $280.00

HRas Antibody

$245.00
SKU: 251442
GTPase HRas (HRas) binds GDP/GTP and possess intrinsic GTPase activity. HRas alternates between an inactive form bound to GDP and an active form bound to GTP. HRas is activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP). Defects in HRAS are the cause of Costello syndrome (COSTS), also known as faciocutaneoskeletal syndrome.
List price: $0.00
Price: $245.00

KRas Antibody

$245.00
SKU: 251405
KRas (K-Ras 2, Ki-Ras, c-K-ras, cKi-ras) binds GDP/GTP and possess intrinsic GTPase activity. Defects in KRas are a cause of acute myelogenous leukemia (AML), a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Defects in KRAS are also a cause of juvenile myelomonocytic leukemia (JMML), Noonan syndrome type 3 (NS3), and cardiofaciocutaneous syndrome (CFC syndrome).
List price: $0.00
Price: $245.00

Tubulin-beta Antibody

$231.00
SKU: 250904
Tubulin is a major cytoskeleton protein that has five distinct forms designated alpha, beta, gamma, delta and epsilon tubulin. The alpha and beta tubulins form a heterodimer that polymerize into the cylindrical microtubule fibers. Both alpha and beta tubulin isomer can be modified by addition of a C-terminal tyrosine residue. This modification may influence polymerization rates. The gamma tubulin isomer is localized to centrosomes which compose the heart of the microtubule organizing center from which microtubule fibers emanate.
List price: $0.00
Price: $231.00

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