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Ubiquitins

SMURF1 Peptide

$50.00
SKU: 350487
SMURF1 is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and directly transfers the ubiquitin to targeted substrates. SMURF1 interacts with receptor-regulated SMADs specific for the BMP pathway, SMAD1 and SMAD5, in order to trigger their ubiquitination and degradation.
List price: $0.00
Storage 
Store at -20°C. The product is hygroscopic and must be protected from light. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C.
Price: $50.00
Protein Family 
Enzymes
Ubiquitins
Pathway and Disease 
Folding
Sorting and Degradation
Signal Transduction
Format 
Each vial contains 0.1 mg of lyophilized peptide. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml. Use at 5.6 ug/ml for a 100X excess over antibody for maximum blocking effect.
Alternate Names 
Smad ubiquitination regulatory factor 1; E3 ubiquitin-protein ligase SMURF1; Smad-specific E3 ubiquitin ligase 1; hSMURF1
Composition 
C72H120N26O25S1
Sequence 
The synthetic peptide used to raise the antibody Cat. No. 200125 is selected from a sequence within the center region of human SMURF1. For blocking experiments, a 10 to 100 fold molar excess to antibody is recommended.
Purity 
Purity > 80% by HPLC
Solubility 
Distilled water for a solution up to 2 mg/ml, otherwise we recommend using acetonitrile.
Release Date 
Monday, December 17, 2012
Subcategory 
Synthetic Peptide
Blocking Peptide
MW 
1781.9 g/mol
Unit 
0.1 mg
Accession No.  
Q9HCE7

SYVN1 Peptide

$50.00
SKU: 350470
Synoviolin (SYVN1) acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates subsequently promoting their degradation. For instance, SYVN1 protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. SYVN1 is up-regulated in synovial tissues from patients with rheumatoid arthritis.
List price: $0.00
Storage 
Store at -20°C. The product is hygroscopic and must be protected from light. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C.
Price: $50.00
Protein Family 
Enzymes
Ubiquitins
Pathway and Disease 
Folding
Sorting and Degradation
Format 
Each vial contains 0.1 mg of lyophilized peptide. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml. Use at 5.6 ug/ml for a 100X excess over antibody for maximum blocking effect.
Alternate Names 
E3 ubiquitin-protein ligase synoviolin; Synovial apoptosis inhibitor 1; HRD1; SYVN1
Composition 
C81H130N24O34S2
Sequence 
The synthetic peptide used to raise the antibody Cat. No. 200108 is selected from a sequence within the C-term region of human SYVN1. For blocking experiments, a 10 to 100 fold molar excess to antibody is recommended.
Purity 
Purity > 80% by HPLC
Solubility 
Distilled water for a solution up to 2 mg/ml, otherwise we recommend using acetonitrile.
Release Date 
Monday, December 17, 2012
Subcategory 
Synthetic Peptide
Blocking Peptide
MW 
2048.1 g/mol
Unit 
0.1 mg
Accession No.  
Q86TM6

ACADM 293 Cell Lysate

$392.00
SKU: 400100
Antigen standard for acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain (ACADM), nuclear gene encoding mitochondrial protein, transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
List price: $0.00
Subcategory 
Cell Lysate
Price: $392.00
Protein Family 
Chaperones and Folding Catalysts
Enzymes
Ubiquitins
Pathway and Disease 
Folding
Sorting and Degradation
Format 
This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Applications 
ELISA
WB
IP
Alternate Names 
isoform a precursor is encoded by transcript variant 1; medium-chain specific acyl-CoA dehydrogenase, mitochondrial; ACADM; ACAD1; FLJ18227; FLJ93013; FLJ99884; MCAD; MCADH
Application Notes 
WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Storage 
Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Release Date 
Thursday, December 13, 2012
Unit 
0.1 mg
Accession No.  
NP_000007

PIAS2 293 Cell Lysate

$672.00
SKU: 401351
Antigen standard for protein inhibitor of activated STAT, 2 (PIAS2), transcript variant beta is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC212659, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
List price: $0.00
Image 
PIAS2 lysate validation: The PIAS2 293 Cell Lysate (lane B) and Control Vector Cell Lysate (lane A) are used in Western blot to detect tagged-PIAS2 with a DDK Antibody.
Subcategory 
Cell Lysate
Price: $672.00
Protein Family 
Enzymes
Ubiquitins
Pathway and Disease 
Cancers
Folding
Sorting and Degradation
Infectious Diseases
Signal Transduction
ORF Clone URL 
http://www.origene.com/orf_clone/trueclone/accession/NM_004671/RC212659.aspx
Format 
This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Applications 
ELISA
WB
IP
Alternate Names 
Msx-interacting-zinc finger; zinc finger, MIZ-type containing 4; DIP; ARIP3; PIAS-NY protein; DAB2-interacting protein; msx-interacting zinc finger protein; protein inhibitor of activated STAT x; androgen receptor-interacting protein 3; PIAS2; MGC102682; miz; MIZ1; PIASX; PIASX-ALPHA; PIASX-BETA; SIZ2; ZMIZ4; E3 SUMO-protein ligase PIAS2
Application Notes 
WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Storage 
Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Release Date 
Thursday, December 13, 2012
Unit 
0.1 mg
Accession No.  
NP_004662

ZBTB7A Antibody

$265.00
SKU: 254411

The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. ZBTB7A, also known as LRF, was initially identified as a potential target of the LAZ-3/BCL-6 oncogene. Later reports demonstrated that it could bind to the wild-type IST (inducer of short transcripts) elements of HIV-1 long terminal repeats (LTRs) and stimulate Tat activity on the HIV-1 LTR.

Western blot analysis of ZBTB7A in human ovary tissue lysate with ZBTB7A Antibody at (A) 1 and (B) 2 ug/ml.
ZBTB7A staining in ovary. Paraffin-embedded human ovary is stained with ZBTB7A Antibody (Cat. No. 254411) used at 1:100 dilution.
List price: $0.00
Price: $265.00

KLHL1 (3A8) Antibody

$308.00
SKU: 253069
KLHL1 (Kelch-like protein 1) plays a role in organizing the actin cytoskeleton of the brain cells. The transcriptional regulation of KLHL1 has been lined to spinocerebellar ataxia (SCA) , a progressive, degenerative genetic disease with multiple types, each of which could be considered a disease in its own right.
List price: $0.00
Price: $308.00

CDC27 (5C12) Antibody

$308.00
SKU: 253024
Cdc27 (Cell division cycle protein 27 homolog) is a component of the Anaphase Promoting Complex (APC). APC is composed of eight protein subunits and is highly conserved in eucaryotic cells. The APC catalyzes the formation of the cyclin B ubiquitin conjugate that is responsible for the ubiquitin mediated proteolysis of B type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein protein interaction.
List price: $0.00
Price: $308.00

SKP2 (6G9D) Antibody

$308.00
SKU: 252839
Skp2 (S-phase kinase-associated protein 2) is a substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Skp2 specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition.
List price: $0.00
Price: $308.00

Parkin (Ser378) Antibody

$350.00
SKU: 252571
Parkin is an E3 ligase in the ubiquitin-proteasome system. Hereditary Parkinson’s disease is most commonly caused by mutations in the parkin gene and is characterized by the progressive loss of dopaminergic neurons and the presence of Lewy bodies in the substania nigra. Recent evidence suggests that phosphorylation of parkin at Ser378 may have an important regulatory role on its E3 ubiquitin ligase activity.
List price: $0.00
Price: $350.00

Parkin (Ser101) Antibody

$350.00
SKU: 252570
Parkin is an E3 ligase in the ubiquitin-proteasome system. Hereditary Parkinson’s disease is most commonly caused by mutations in the parkin gene and is characterized by the progressive loss of dopaminergic neurons and the presence of Lewy bodies in the substania nigra. Recent evidence suggests that phosphorylation of parkin at Ser101 may have an important regulatory role on its E3 ubiquitin ligase activity.
List price: $0.00
Price: $350.00

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