Human CNTF

Recombinant Active Protein

Cat. No.

600104

Unit

0.02 mg

Price $235.00

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Abbiotec

7985 Dunbrook Rd., Ste A
San Diego, CA 92126, USA

Phone: 1 858 586 0500,
1 800 854 7453

Fax: 1 858 586 6252

Email: info@abbiotec.com

Website: http://www.abbiotec.com

Description

Ciliary neurotrophic factor (CNTF) is a neurotrophic factor that promotes the survival of various neuronal cell types and may play an important role in the injury response in the nervous system. CNTF, like FGF acidic, FGF basic, and PD-ECGF (platelet-derived endothelial cell growth factor ), does not possess a signal sequence that would allow secretion of the factor by classical secretion pathways (endoplasmatic reticulum/Golgi system). The mechanism underlying the release of CNTF is unknown. Recombinant Human CNTF produced in E. coli is a non-glycosylated single polypeptide chain containing 199 amino acids with a MW of 22,706 Da.

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Datasheet

Format

Each vial contains 20 ug protein lyophilized in 10 mM sodium phosphate pH7.5. Reconstitute with 0.2 ml sterile deionized water for a final concentration of 0.1 mg/ml.

Alternate Names

Ciliary neurotrophic factor; CNTF

Accession No.

P26441

22706 Da

Sequence

MAFTEHSPLT PHRRDLCSRS IWLARKLRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM

Source

E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Purity

Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Application Notes

Recombinant Human Ciliary Neurotrophic Factor is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of TF-1 cells is less then 1.8 ng/ml.

Storage

Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.

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For research use only, not for diagnostic or therapeutic procedures.

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Human CNTF

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