Rat IL-6

Recombinant Active Protein

Cat. No.

600224

Unit

0.01 mg

Price $250.00

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Abbiotec

7985 Dunbrook Rd., Ste A
San Diego, CA 92126, USA

Phone: 1 858 586 0500 x100,
1 800 854 7453

Fax: 1 858 586 6252

Email: info@abbiotec.com

Website: http://www.abbiotec.com

Description

Interleukin (IL)-6 is an important proinflammatory and immunoregulatory cytokine. It is expressed by various cells and .has been shown to inhibit the growth of early stage melanoma cells in vitro. In contrast, it promotes the proliferation of advanced stage melanoma cells in vitro. Recombinant Rat IL-6 produced in E. coli is a single non-glycosylated polypeptide chain containing 187 amino acids with a MW of 21,732 Da.

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Datasheet

Format

Each vial contains 10 ug protein lyophilized in 0.1% TFA. Reconstitute with 0.1 ml sterile 10 mM HCl for a final concentration of 0.1 mg/ml.

Alternate Names

interleukin-6; IL-6; b-cell stimulatory factor 2; BSF-2; interferon beta-2; hybridoma growth factor; CTL differentiation factor; CDF

Accession No.

P20607

21732 Da

Sequence

MFPTSQVRRG DFTEDTTHNR PVYTTSQVGG LITYVLREIF EMRKELCNGN SDCMNSDDAL SENNLKLPEI QRNDGCFQTG YNQEICLLKI CSGLLEFRFY LEFVKNNLQD NKKDKARVIQ SNTETLVHIF KQEIKDSYKI VLPTPTSNAL LMEKLESQKE WLRTKTIQLI LKALEEFLKV TMRSTRQT

Source

E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.

Purity

Purity > 90% as determined by reducing and non-reducing SDS-PAGE. Protein Content determined by quantitation on SDS-PAGE against a known standard.

Application Notes

Recombinant Rat IL-6 is fully biologically active when compared to standard. A dose-dependent stimulation of mouse 7TD1 cells showed an ED50 of < 0.01 ng/ml.

Storage

Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.

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For research use only, not for diagnostic or therapeutic procedures.

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Rat IL-6

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