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ZNHIT3 293 Cell Lysate

Cell Lysate

Unit

0.1 mg

Cat. No.

408319

Price $392.00

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938 S. Andreasen Dr., Ste I
Escondido, CA 92029, USA

Phone: 1 858 586 0500 x100,
1 800 854 7453

Fax: 1 858 586 6252

Email: info@abbiotec.com

Website: http://www.abbiotec.com

Description

Antigen standard for zinc finger, HIT type 3 (ZNHIT3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.

Spec Container

Datasheet

Applications

ELISA

Format

This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).

Alternate Names

thyroid receptor interacting protein 3; thyroid hormone receptor interactor 3; zinc finger, HIT domain containing 3; TRIP-3; HNF-4a coactivator; thyroid receptor-interacting protein 3; ZNHIT3; TRIP3

Accession No.

NP_004764

Application Notes

WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Storage

Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.

Images

For research use only, not for diagnostic or therapeutic procedures.

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ZNHIT3 293 Cell Lysate

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