Rat GDNF

Recombinant Active Protein

Cat. No.

600378

Unit

0.01 mg

Price $250.00

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Abbiotec

938 S. Andreasen Dr., Ste I
Escondido, CA 92029, USA

Phone: 1 858 586 0500 x100,
1 800 854 7453

Fax: 1 858 586 6252

Email: info@abbiotec.com

Website: http://www.abbiotec.com

Description

Glial Cell Line-Derived Neurotrophic Factor, or GDNF, is a neurotrophic factor that is closely related to other neurotrophic factors such as Neurturin, Persephin, and Artemin, by a common structural feature called the cysteine-knot. GDNF signals through a multicomponent system of receptors that includes receptors known as RET, and GFR alpha 1-4 to promote dopamine uptake, survival and differentiation of neurons. Rat GDNF is produced in E.coli. Recombinant rat GDNF is a non-glycosylated homodimer, containing two 135 amino acid chains, with a total molecular weight of 30.1 kDa.

Spec Container

Datasheet

Format

Each vial contains 10 ug of lyophilized protein. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.

Alternate Names

Glial cell line-derived neurotrophic factor; astrocyte-derived trophic factor; ATF; GDNF

Accession No.

Q07731

30100 Da

Sequence

MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCEAAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDSLV YHILRKHSAK RCGCI

Source

E. coli. Endotoxin level as measured by LAL is <0.05ng/ug or <0.5EU/ug.

Purity

Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Application Notes

The activity is determined by the dose-dependent proliferation of C6 cells and is typically less than 0.1 ug/mL. Specific activity: 1 x 10e4 units/mg

Storage

Store at -20C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.

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For research use only, not for diagnostic or therapeutic procedures.

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Rat GDNF

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