Ph. 858 586 0500    Toll Free 800 854 7453

Custom Antibody Production

Abbiotec offers custom antibody production services to the scientific community to accelerate a project From Biology to Discovery™. When the antibody for a chosen antigen is not already available off the shelf, it is time to develop a custom project. The experienced staff of Abbiotec processes a custom project with the same mindset and tools that it develops antibody products for the Abbiotec catalog: we want an antibody that specifically detects the antigen in the chosen application.

Polyclonal Antibody Production Services

We are proud of offering “Made in USA” quality label for these services by using our USDA-registered animal facility. Abbiotec has simplified the ordering process by offering 3 packages combining the most common customer requests:

Protein-specific Ab Combo: With 3 mg of protein as antigen, 100-200 mg of purified antibody is generated in rabbits against multiple epitopes within 3 months.

Peptide-specific Ab Combo: A peptide encompassing a short region of the target protein is used as antigen to generate in rabbits 100-200 mg of purified antibody within 4 months.

Phospho-specific Ab Combo: For monitoring target activation by phosphorylation, a phosphopeptide is used as antigen to generate in rabbits 5-10 mg of affinity-purified antibody within 4 months.

Abbiotec offers complimentary peptide design services to ensure the success of the project.

Cat. No.

100100

100200

100300

Polyclonal Antibody Combo

Protein-specific Ab

Peptide-specific Ab

Phospho-specific Ab

Immunogen

Protein

Peptide

phospho-peptide

Peptide synthesis, purity>70%

Peptide synthesis, purity>90%

Optional

Peptide-KLH conjugation

Immunization and sera collection

Titration by ELISA

Protein-A purification

Antigen-affinity purification

Optional

Optional

Time

3 months

4 months

4 months

Price

$1,000.00

$1,500.00

$3,000.00

Abbiotec offers numerous other antibody production services to customize a project to the investigator’s request. A non-exhaustive list is displayed below. For more information, please contact Technical Service Dept.

Cat. No.

Polyclonal Antibody Services

Price

100100

Protein-specific Ab Combo

Cat No. 100120, 100130, 100150

$1,000.00

100200

Peptide-specific Ab Combo

Cat No. 100210, 100110, 100120, 100130, 100150

$1,500.00

100300

Phospho-specific Ab Combo

Cat No. 100220, 100110, 100120, 100130, 100320

$3,000.00

100210

Peptide synthesis, purity>70%, 15-19 mg

$300.00

100220

Peptide synthesis, purity>90%, 15-19 mg

$500.00

100310

Phospho-peptide synthesis, purity>90%, 15-19 mg

$800.00

100110

Peptide-carrier bioconjugation

$200.00

100120

Animal immunization and antisera production (2 rabbits)

$600.00

100130

Titer test by ELISA

$100.00

100140

SAS purification

$200.00

100150

Protein-A purification

$300.00

100160

Antigen-affinity purification

$500.00

100320

2-step phospho-specific purification by peptide affinity

$1,000.00

Monoclonal Antibody Production Options

Abbiotec offers different options to develop a monoclonal antibody with preset properties. Proteins, peptides, modified peptides, or cells can be used as antigens in mice and rats.

Protein: With 2 mg of protein as antigen, we generate a set of monoclonal antibodies against the target protein for assay development. We purified 10 mg of antibody within 5 months.

Peptide: We produce clones against a short region of the target protein. Abbiotec designs and synthesizes the best peptide candidate from the protein sequence. We purify 10 mg of purified antibody within 6 months.

Modification-containing peptide: For monitoring target activation by phosphorylation for instance, a phosphopeptide is used as antigen to generate phosphospecific monoclonal antibodies. We purify 2-10 mg of antibody within 6 months.

Abbiotec offers complimentary peptide design services to ensure the success of the project.

Cat. No.

Monoclonal Antibody Services

Price

Protein mAb Combo

Cat No. 102100, 102200, 102300

$6,100.00

Peptide mAb Combo

Cat No. 100220, 100110, 102100, 102200, 102300

$6,800.00

Modified Peptide mAb Combo

Cat No. 100310, 100220, 100110, 102100, 102200, 102300

$7,600.00

100220

Peptide synthesis up to 20 aa, purity>90%, 15-20 mg

$500.00

100310

Phospho-peptide synthesis up to 20 aa, purity>90%, 15-20 mg

$800.00

100110

Peptide-carrier bioconjugation

$200.00

102100

Animal immunization (5 mice), serum titration and spleen collection

$1,500.00

102200

Hybridoma fusion, screening & subculture

$3,600.00

102300

Antibody Production & Purification (2 clones up to 15 mg)

$1,000.00

Get a Quotation

Since prices vary upon project customization, we recommend submitting a quotation using the Quote Form for obtaining an accurate price. All requests are processed within 48 hrs.

Guarantees

Abbiotec guarantees competitive pricing and delivery time for all routine options.

Every antibody project is supplied with a Peptide Design and Project reports, when applicable. Abbiotec guarantees that the antibody generated using an antigen synthesized by Abbiotec will recognize the antigen in ELISA.

Althought most projects can be completed within 3-6 months, actual processing time is dependent on the peptide synthesis, animal immunization processes and clone subculture. Please contact the Technical Service Dept for updated timelines. Estimated delivery times are stated on all quotations.