Abbiotec offers custom antibody production services to the scientific community to accelerate a project From Biology to Discovery™. When the antibody for a chosen antigen is not already available off the shelf, it is time to develop a custom project. The experienced staff of Abbiotec processes a custom project with the same mindset and tools that it develops antibody products for the Abbiotec catalog: we want an antibody that specifically detects the antigen in the chosen application.
Polyclonal Antibody Production Services
We are proud of offering “Made in USA” quality label for these services by using our USDA-registered animal facility. Abbiotec has simplified the ordering process by offering 3 packages combining the most common customer requests:
Protein-specific Ab Combo: With 3 mg of protein as antigen, 100-200 mg of purified antibody is generated in rabbits against multiple epitopes within 3 months.
Peptide-specific Ab Combo: A peptide encompassing a short region of the target protein is used as antigen to generate in rabbits 100-200 mg of purified antibody within 4 months.
Phospho-specific Ab Combo: For monitoring target activation by phosphorylation, a phosphopeptide is used as antigen to generate in rabbits 5-10 mg of affinity-purified antibody within 4 months.
Abbiotec offers complimentary peptide design services to ensure the success of the project.
|
Cat. No. |
100100 |
100200 |
100300 |
|---|---|---|---|
|
Polyclonal Antibody Combo |
Protein-specific Ab |
Peptide-specific Ab |
Phospho-specific Ab |
|
Immunogen |
Protein |
Peptide |
phospho-peptide |
|
Peptide synthesis, purity>70% |
√ |
||
|
Peptide synthesis, purity>90% |
Optional |
√ |
|
|
Peptide-KLH conjugation |
√ |
√ |
|
|
Immunization and sera collection |
√ |
√ |
√ |
|
Titration by ELISA |
√ |
√ |
√ |
|
Protein-A purification |
√ |
√ |
|
|
Antigen-affinity purification |
Optional |
Optional |
√ |
|
Time |
3 months |
4 months |
4 months |
|
Price |
$1,000.00 |
$1,500.00 |
$3,000.00 |
Abbiotec offers numerous other antibody production services to customize a project to the investigator’s request. A non-exhaustive list is displayed below. For more information, please contact Technical Service Dept.
|
Cat. No. |
Polyclonal Antibody Services |
Price |
|---|---|---|
|
100100 |
Protein-specific Ab Combo Cat No. 100120, 100130, 100150 |
$1,000.00 |
|
100200 |
Peptide-specific Ab Combo Cat No. 100210, 100110, 100120, 100130, 100150 |
$1,500.00 |
|
100300 |
Phospho-specific Ab Combo Cat No. 100220, 100110, 100120, 100130, 100320 |
$3,000.00 |
|
100210 |
Peptide synthesis, purity>70%, 15-19 mg |
$300.00 |
|
100220 |
Peptide synthesis, purity>90%, 15-19 mg |
$500.00 |
|
100310 |
Phospho-peptide synthesis, purity>90%, 15-19 mg |
$800.00 |
|
100110 |
Peptide-carrier bioconjugation |
$200.00 |
|
100120 |
Animal immunization and antisera production (2 rabbits) |
$600.00 |
|
100130 |
Titer test by ELISA |
$100.00 |
|
100140 |
SAS purification |
$200.00 |
|
100150 |
Protein-A purification |
$300.00 |
|
100160 |
Antigen-affinity purification |
$500.00 |
|
100320 |
2-step phospho-specific purification by peptide affinity |
$1,000.00 |
Monoclonal Antibody Production Options
Abbiotec offers different options to develop a monoclonal antibody with preset properties. Proteins, peptides, modified peptides, or cells can be used as antigens in mice and rats.
Protein: With 2 mg of protein as antigen, we generate a set of monoclonal antibodies against the target protein for assay development. We purified 10 mg of antibody within 5 months.
Peptide: We produce clones against a short region of the target protein. Abbiotec designs and synthesizes the best peptide candidate from the protein sequence. We purify 10 mg of purified antibody within 6 months.
Modification-containing peptide: For monitoring target activation by phosphorylation for instance, a phosphopeptide is used as antigen to generate phosphospecific monoclonal antibodies. We purify 2-10 mg of antibody within 6 months.
Abbiotec offers complimentary peptide design services to ensure the success of the project.
|
Cat. No. |
Monoclonal Antibody Services |
Price |
|---|---|---|
|
Protein mAb Combo Cat No. 102100, 102200, 102300 |
$6,100.00 |
|
|
Peptide mAb Combo Cat No. 100220, 100110, 102100, 102200, 102300 |
$6,800.00 |
|
|
Modified Peptide mAb Combo Cat No. 100310, 100220, 100110, 102100, 102200, 102300 |
$7,600.00 |
|
|
100220 |
Peptide synthesis up to 20 aa, purity>90%, 15-20 mg |
$500.00 |
|
100310 |
Phospho-peptide synthesis up to 20 aa, purity>90%, 15-20 mg |
$800.00 |
|
100110 |
Peptide-carrier bioconjugation |
$200.00 |
|
102100 |
Animal immunization (5 mice), serum titration and spleen collection |
$1,500.00 |
|
102200 |
Hybridoma fusion, screening & subculture |
$3,600.00 |
|
102300 |
Antibody Production & Purification (2 clones up to 15 mg) |
$1,000.00 |
Get a Quotation
Since prices vary upon project customization, we recommend submitting a quotation using the Quote Form for obtaining an accurate price. All requests are processed within 48 hrs.
Guarantees
Abbiotec guarantees competitive pricing and delivery time for all routine options.
Every antibody project is supplied with a Peptide Design and Project reports, when applicable. Abbiotec guarantees that the antibody generated using an antigen synthesized by Abbiotec will recognize the antigen in ELISA.
Althought most projects can be completed within 3-6 months, actual processing time is dependent on the peptide synthesis, animal immunization processes and clone subculture. Please contact the Technical Service Dept for updated timelines. Estimated delivery times are stated on all quotations.